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Cosmo Genetech Co abi 3711 automatic sequencer
Abi 3711 Automatic Sequencer, supplied by Cosmo Genetech Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/abi+3711+automatic+sequencer/abi+3711+automatic+sequencer/pmc07913755-52-55-58
Average 90 stars, based on 1 article reviews
abi 3711 automatic sequencer - by Bioz Stars, 2026-10
90/100 stars

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Polymerase Chain Reaction:

Article Title: Optimized Detoxification of a Live Attenuated Vaccine Strain (SG9R) to Improve Vaccine Strategy against Fowl Typhoid
Article Snippet: Bacterial genomic DNA was extracted using the G-spin Genomic DNA Extraction Kit for Bacteria (iNtRON Biotechnology, Seongnam-si, Korea) and PCR was conducted under the following conditions: 1 μL of the template DNA (50 ng/μL), 3 μL of 10 × buffer, 3 μL of dNTPs (5 mM), 0.5 μL of each primer (10 pmol/μL), and 0.25 μL of Taq polymerase (MGmed, Seoul, Korea). .. The thermal cycling conditions were as follows: 95 °C for 5 min, followed by 35 cycles at 95 °C for 30 s, 55 °C for 30 s, 72 °C for 1 min, and 72 °C for 5 min. PCR amplicons were purified using a PCR/Gel Purification Kit (MGmed), and sequencing was performed using the ABI 3711 automatic sequencer (Cosmogenetech, Seoul, Korea). .. Nucleotide sequences were translated and compared using the BioEdit program version 7.2.5.

Purification:

Article Title: Optimized Detoxification of a Live Attenuated Vaccine Strain (SG9R) to Improve Vaccine Strategy against Fowl Typhoid
Article Snippet: Bacterial genomic DNA was extracted using the G-spin Genomic DNA Extraction Kit for Bacteria (iNtRON Biotechnology, Seongnam-si, Korea) and PCR was conducted under the following conditions: 1 μL of the template DNA (50 ng/μL), 3 μL of 10 × buffer, 3 μL of dNTPs (5 mM), 0.5 μL of each primer (10 pmol/μL), and 0.25 μL of Taq polymerase (MGmed, Seoul, Korea). .. The thermal cycling conditions were as follows: 95 °C for 5 min, followed by 35 cycles at 95 °C for 30 s, 55 °C for 30 s, 72 °C for 1 min, and 72 °C for 5 min. PCR amplicons were purified using a PCR/Gel Purification Kit (MGmed), and sequencing was performed using the ABI 3711 automatic sequencer (Cosmogenetech, Seoul, Korea). .. Nucleotide sequences were translated and compared using the BioEdit program version 7.2.5.

Sequencing:

Article Title: Optimized Detoxification of a Live Attenuated Vaccine Strain (SG9R) to Improve Vaccine Strategy against Fowl Typhoid
Article Snippet: Bacterial genomic DNA was extracted using the G-spin Genomic DNA Extraction Kit for Bacteria (iNtRON Biotechnology, Seongnam-si, Korea) and PCR was conducted under the following conditions: 1 μL of the template DNA (50 ng/μL), 3 μL of 10 × buffer, 3 μL of dNTPs (5 mM), 0.5 μL of each primer (10 pmol/μL), and 0.25 μL of Taq polymerase (MGmed, Seoul, Korea). .. The thermal cycling conditions were as follows: 95 °C for 5 min, followed by 35 cycles at 95 °C for 30 s, 55 °C for 30 s, 72 °C for 1 min, and 72 °C for 5 min. PCR amplicons were purified using a PCR/Gel Purification Kit (MGmed), and sequencing was performed using the ABI 3711 automatic sequencer (Cosmogenetech, Seoul, Korea). .. Nucleotide sequences were translated and compared using the BioEdit program version 7.2.5.



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